Velocity Measurement Tool

The toolset allows to create kymograms and to measure the velocity of particles in the kymogram. The user can make a segmented line selection on the kymogram and the mean velocity for each segment will be measured. A synthetic example image can be found here: moving-particles-2.tif

Getting started

To install the tools, drag the link Velocity_Measurement_Tool.txt to the ImageJ launcher window, save it under macros/toolsets in the ImageJ installation and restart ImageJ.

Select the "Velocity Measurement Tool" toolset from the >> button of the ImageJ launcher.

  • the *?*-button opens this help page
  • the k-button creates a kymogram of the current image
  • the v-button measures the velocities corresponding to the line or segmented line selection on the kymogram

The measurement of the velocities is a modified version of code from www.embl.de/emnet/html/kymograph.html (tsp050706.txt TimeSpacePlot (Kymograph)) by J. Rietdorf FMI Basel and A. Seitz EMBL Heidelberg.

Usage

Make a segmented line selection of the path of the particle for which you ant to measure the velocity. You can use a z-projection (Image>Stacks>Z Project...) to create the selection and then transfer it to the time-series stack using ctrl+shift+e. Now press the k-button to get the kymogram. Now make a segmented line selection on the trace of the particle in the kymogram and press the v-button to get the speed measurements.

Results

The input time series: The kymogram:

The kymogram with a segmented line selection:

The measurements in the results table:

  • dy sum - the sum of the distance travelled in y direction
  • dx sum - the sum of the distance travelled in x direction
  • dy now - the distance travelled on the current path segment in y direction
  • dx now - the distance travelled on the current path segment in x direction
  • actual speed - the speed on the current path segment
  • average speed - the average speed upto and including the current path segment

The x-axis represents the distance and the y-axis represents the time in the units set for the image for example pixel/time unit or micron/second.

Publications that use the tool

  1. Sawamiphak, S., Kontarakis, Z., Filosa, A., Reischauer, S., and Stainier, D.Y.R. (2017). Transient cardiomyocyte fusion regulates cardiac development in zebrafish. Nature Communications 8.
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  2. Breznau, E.B., Murt, M., Blasius, T.L., Verhey, K.J., and Miller, A.L. (2017). The MgcRacGAP SxIP motif tethers Centralspindlin to microtubule plus ends in Xenopus laevis. Journal of Cell Science 130, 1809–1821.
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  3. Elaina B. Breznau (2016). Regulation of epithelial cytokinesis and cell-cell junctions by MgcRacGAP. University of Michigan.
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  4. Monteith, C.E., Brunner, M.E., Djagaeva, I., Bielecki, A.M., Deutsch, J.M., and Saxton, W.M. (2016). A Mechanism for Cytoplasmic Streaming: Kinesin-Driven Alignment of Microtubules and Fast Fluid Flows. Biophysical Journal 110, 2053–2065.

moving-particles-2.tif (2.32 MB) Volker Baecker, 04/03/2014 02:08 PM

toolset.png (701 Bytes) Volker Baecker, 04/03/2014 02:13 PM

moving-particles-2.gif (20.2 KB) Volker Baecker, 04/03/2014 02:19 PM

kymogram.png (191 Bytes) Volker Baecker, 04/03/2014 02:21 PM

kymogram-2.png (9.79 KB) Volker Baecker, 04/03/2014 02:25 PM

results.png (7.77 KB) Volker Baecker, 04/03/2014 02:29 PM